One-step purification and characterization of a lignin-specific O-methyltransferase from poplar.
Identifieur interne : 004B75 ( Main/Exploration ); précédent : 004B74; suivant : 004B76One-step purification and characterization of a lignin-specific O-methyltransferase from poplar.
Auteurs : J. Van Doorsselaere [Belgique] ; B. Dumas ; M. Baucher ; B. Fritig ; M. Legrand ; M. Van Montagu ; D. InzéSource :
- Gene [ 0378-1119 ] ; 1993.
Descripteurs français
- KwdFr :
- ARN messager (métabolisme), Arbres (MeSH), Chromatographie d'affinité (MeSH), Clonage moléculaire (MeSH), Escherichia coli (MeSH), Lignine (métabolisme), Methyltransferases (génétique), Methyltransferases (métabolisme), Plasmides (MeSH), Réaction de polymérisation en chaîne (MeSH), Technique de Northern (MeSH), Technique de Southern (MeSH), Électrophorèse sur gel de polyacrylamide (MeSH).
- MESH :
- génétique : Methyltransferases.
- métabolisme : ARN messager, Lignine, Methyltransferases.
- Arbres, Chromatographie d'affinité, Clonage moléculaire, Escherichia coli, Plasmides, Réaction de polymérisation en chaîne, Technique de Northern, Technique de Southern, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Blotting, Northern (MeSH), Blotting, Southern (MeSH), Chromatography, Affinity (MeSH), Cloning, Molecular (MeSH), Electrophoresis, Polyacrylamide Gel (MeSH), Escherichia coli (MeSH), Lignin (metabolism), Methyltransferases (genetics), Methyltransferases (metabolism), Plasmids (MeSH), Polymerase Chain Reaction (MeSH), RNA, Messenger (metabolism), Trees (MeSH).
- MESH :
- chemical , genetics : Methyltransferases.
- chemical , metabolism : Lignin, Methyltransferases, RNA, Messenger.
- Blotting, Northern, Blotting, Southern, Chromatography, Affinity, Cloning, Molecular, Electrophoresis, Polyacrylamide Gel, Escherichia coli, Plasmids, Polymerase Chain Reaction, Trees.
Abstract
O-Methyltransferases (OMT; EC 2.1.1.6) play an important role in the synthesis of lignin precursors by catalyzing the O-methylation of o-diphenolic substrates such as caffeic acid (CA) and 5-hydroxyferulic acid (5OH). Here, we report on the purification of a lignin-specific OMT (38 kDa) from poplar (Populus trichocarpa x P. deltoides). The OMT was purified from xylem by a single affinity chromatography step on adenosine agarose. The enzyme uses both CA and 5OH as substrates. We previously have reported the cloning of a corresponding OMT cDNA [Dumas et al., Plant Physiol. 98 (1992) 796-797]. Expression of this OMT cDNA in Escherichia coli further confirmed the identity of the clone. Genomic hybridization demonstrates the presence of one or two OMT genes per haploid poplar genome. RNA gel blot hybridization shows high levels of steady-state OMT mRNA in the xylem of young poplar trees, as compared to the levels in leaves.
DOI: 10.1016/0378-1119(93)90640-o
PubMed: 8224909
Affiliations:
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Dumas</name></author><author><name sortKey="Baucher, M" sort="Baucher, M" uniqKey="Baucher M" first="M" last="Baucher">M. Baucher</name></author><author><name sortKey="Fritig, B" sort="Fritig, B" uniqKey="Fritig B" first="B" last="Fritig">B. Fritig</name></author><author><name sortKey="Legrand, M" sort="Legrand, M" uniqKey="Legrand M" first="M" last="Legrand">M. Legrand</name></author><author><name sortKey="Van Montagu, M" sort="Van Montagu, M" uniqKey="Van Montagu M" first="M" last="Van Montagu">M. Van Montagu</name></author><author><name sortKey="Inze, D" sort="Inze, D" uniqKey="Inze D" first="D" last="Inzé">D. Inzé</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1993">1993</date><idno type="RBID">pubmed:8224909</idno><idno type="pmid">8224909</idno><idno type="doi">10.1016/0378-1119(93)90640-o</idno><idno type="wicri:Area/Main/Corpus">004B60</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004B60</idno><idno type="wicri:Area/Main/Curation">004B60</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004B60</idno><idno type="wicri:Area/Main/Exploration">004B60</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">One-step purification and characterization of a lignin-specific O-methyltransferase from poplar.</title><author><name sortKey="Van Doorsselaere, J" sort="Van Doorsselaere, J" uniqKey="Van Doorsselaere J" first="J" last="Van Doorsselaere">J. Van Doorsselaere</name><affiliation wicri:level="4"><nlm:affiliation>Laboratorium voor Genetica, Universiteit Gent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Laboratorium voor Genetica, Universiteit Gent</wicri:regionArea><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Dumas, B" sort="Dumas, B" uniqKey="Dumas B" first="B" last="Dumas">B. Dumas</name></author><author><name sortKey="Baucher, M" sort="Baucher, M" uniqKey="Baucher M" first="M" last="Baucher">M. Baucher</name></author><author><name sortKey="Fritig, B" sort="Fritig, B" uniqKey="Fritig B" first="B" last="Fritig">B. Fritig</name></author><author><name sortKey="Legrand, M" sort="Legrand, M" uniqKey="Legrand M" first="M" last="Legrand">M. Legrand</name></author><author><name sortKey="Van Montagu, M" sort="Van Montagu, M" uniqKey="Van Montagu M" first="M" last="Van Montagu">M. Van Montagu</name></author><author><name sortKey="Inze, D" sort="Inze, D" uniqKey="Inze D" first="D" last="Inzé">D. Inzé</name></author></analytic><series><title level="j">Gene</title><idno type="ISSN">0378-1119</idno><imprint><date when="1993" type="published">1993</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Blotting, Northern (MeSH)</term><term>Blotting, Southern (MeSH)</term><term>Chromatography, Affinity (MeSH)</term><term>Cloning, Molecular (MeSH)</term><term>Electrophoresis, Polyacrylamide Gel (MeSH)</term><term>Escherichia coli (MeSH)</term><term>Lignin (metabolism)</term><term>Methyltransferases (genetics)</term><term>Methyltransferases (metabolism)</term><term>Plasmids (MeSH)</term><term>Polymerase Chain Reaction (MeSH)</term><term>RNA, Messenger (metabolism)</term><term>Trees (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (métabolisme)</term><term>Arbres (MeSH)</term><term>Chromatographie d'affinité (MeSH)</term><term>Clonage moléculaire (MeSH)</term><term>Escherichia coli (MeSH)</term><term>Lignine (métabolisme)</term><term>Methyltransferases (génétique)</term><term>Methyltransferases (métabolisme)</term><term>Plasmides (MeSH)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Technique de Northern (MeSH)</term><term>Technique de Southern (MeSH)</term><term>Électrophorèse sur gel de polyacrylamide (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Methyltransferases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term><term>Methyltransferases</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Methyltransferases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term><term>Lignine</term><term>Methyltransferases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Northern</term><term>Blotting, Southern</term><term>Chromatography, Affinity</term><term>Cloning, Molecular</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Escherichia coli</term><term>Plasmids</term><term>Polymerase Chain Reaction</term><term>Trees</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Arbres</term><term>Chromatographie d'affinité</term><term>Clonage moléculaire</term><term>Escherichia coli</term><term>Plasmides</term><term>Réaction de polymérisation en chaîne</term><term>Technique de Northern</term><term>Technique de Southern</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">O-Methyltransferases (OMT; EC 2.1.1.6) play an important role in the synthesis of lignin precursors by catalyzing the O-methylation of o-diphenolic substrates such as caffeic acid (CA) and 5-hydroxyferulic acid (5OH). Here, we report on the purification of a lignin-specific OMT (38 kDa) from poplar (Populus trichocarpa x P. deltoides). The OMT was purified from xylem by a single affinity chromatography step on adenosine agarose. The enzyme uses both CA and 5OH as substrates. We previously have reported the cloning of a corresponding OMT cDNA [Dumas et al., Plant Physiol. 98 (1992) 796-797]. Expression of this OMT cDNA in Escherichia coli further confirmed the identity of the clone. Genomic hybridization demonstrates the presence of one or two OMT genes per haploid poplar genome. RNA gel blot hybridization shows high levels of steady-state OMT mRNA in the xylem of young poplar trees, as compared to the levels in leaves.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8224909</PMID><DateCompleted><Year>1993</Year><Month>12</Month><Day>22</Day></DateCompleted><DateRevised><Year>2019</Year><Month>07</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0378-1119</ISSN><JournalIssue CitedMedium="Print"><Volume>133</Volume><Issue>2</Issue><PubDate><Year>1993</Year><Month>Nov</Month><Day>15</Day></PubDate></JournalIssue><Title>Gene</Title><ISOAbbreviation>Gene</ISOAbbreviation></Journal><ArticleTitle>One-step purification and characterization of a lignin-specific O-methyltransferase from poplar.</ArticleTitle><Pagination><MedlinePgn>213-7</MedlinePgn></Pagination><Abstract><AbstractText>O-Methyltransferases (OMT; EC 2.1.1.6) play an important role in the synthesis of lignin precursors by catalyzing the O-methylation of o-diphenolic substrates such as caffeic acid (CA) and 5-hydroxyferulic acid (5OH). Here, we report on the purification of a lignin-specific OMT (38 kDa) from poplar (Populus trichocarpa x P. deltoides). The OMT was purified from xylem by a single affinity chromatography step on adenosine agarose. The enzyme uses both CA and 5OH as substrates. We previously have reported the cloning of a corresponding OMT cDNA [Dumas et al., Plant Physiol. 98 (1992) 796-797]. Expression of this OMT cDNA in Escherichia coli further confirmed the identity of the clone. Genomic hybridization demonstrates the presence of one or two OMT genes per haploid poplar genome. RNA gel blot hybridization shows high levels of steady-state OMT mRNA in the xylem of young poplar trees, as compared to the levels in leaves.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Van Doorsselaere</LastName><ForeName>J</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Laboratorium voor Genetica, Universiteit Gent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dumas</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Baucher</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Fritig</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Legrand</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Van Montagu</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Inzé</LastName><ForeName>D</ForeName><Initials>D</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Gene</MedlineTA><NlmUniqueID>7706761</NlmUniqueID><ISSNLinking>0378-1119</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.1.1.-</RegistryNumber><NameOfSubstance UI="D008780">Methyltransferases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><GeneSymbolList><GeneSymbol>OMT</GeneSymbol></GeneSymbolList><MeshHeadingList><MeshHeading><DescriptorName UI="D015152" MajorTopicYN="N">Blotting, Northern</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015139" MajorTopicYN="N">Blotting, Southern</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002846" MajorTopicYN="N">Chromatography, Affinity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008780" MajorTopicYN="N">Methyltransferases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1993</Year><Month>11</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1993</Year><Month>11</Month><Day>15</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1993</Year><Month>11</Month><Day>15</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">8224909</ArticleId><ArticleId IdType="pii">0378-1119(93)90640-O</ArticleId><ArticleId IdType="doi">10.1016/0378-1119(93)90640-o</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li></country><region><li>Province de Flandre-Orientale</li><li>Région flamande</li></region><settlement><li>Gand</li></settlement><orgName><li>Université de Gand</li></orgName></list><tree><noCountry><name sortKey="Baucher, M" sort="Baucher, M" uniqKey="Baucher M" first="M" last="Baucher">M. Baucher</name><name sortKey="Dumas, B" sort="Dumas, B" uniqKey="Dumas B" first="B" last="Dumas">B. Dumas</name><name sortKey="Fritig, B" sort="Fritig, B" uniqKey="Fritig B" first="B" last="Fritig">B. Fritig</name><name sortKey="Inze, D" sort="Inze, D" uniqKey="Inze D" first="D" last="Inzé">D. Inzé</name><name sortKey="Legrand, M" sort="Legrand, M" uniqKey="Legrand M" first="M" last="Legrand">M. Legrand</name><name sortKey="Van Montagu, M" sort="Van Montagu, M" uniqKey="Van Montagu M" first="M" last="Van Montagu">M. Van Montagu</name></noCountry><country name="Belgique"><region name="Région flamande"><name sortKey="Van Doorsselaere, J" sort="Van Doorsselaere, J" uniqKey="Van Doorsselaere J" first="J" last="Van Doorsselaere">J. Van Doorsselaere</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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